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dna fragment encoding 2ms2mut  (Thermo Fisher)


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    Thermo Fisher dna fragment encoding 2ms2mut
    Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of <t>MS2-responsive</t> ribozyme switches in human cells for unlocalized <t>(2MS2mut-DsRed),</t> nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.
    Dna Fragment Encoding 2ms2mut, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+fragment+encoding+2ms2mut/DNA/pmc04231745-77-1-13
    Average 99 stars, based on 1 article reviews
    dna fragment encoding 2ms2mut - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Protein-responsive ribozyme switches in eukaryotic cells"

    Article Title: Protein-responsive ribozyme switches in eukaryotic cells

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gku875

    Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of MS2-responsive ribozyme switches in human cells for unlocalized (2MS2mut-DsRed), nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.
    Figure Legend Snippet: Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of MS2-responsive ribozyme switches in human cells for unlocalized (2MS2mut-DsRed), nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.

    Techniques Used: Activity Assay, Control, Concentration Assay, Stable Transfection, Construct, Expressing

    Related Articles

    Synthesized:

    Article Title: Protein-responsive ribozyme switches in eukaryotic cells
    Article Snippet: .. A DNA fragment encoding 2MS2mut (MS2 V75E/A81G head-to-tail fused dimer) was synthesized by GeneArt (Life Technologies) and inserted into pCS2595 between NotI/ApaI to form pCS2686. ..



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    dna fragment encoding 2ms2mut  (Thermo Fisher)
    99
    Thermo Fisher dna fragment encoding 2ms2mut
    Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of <t>MS2-responsive</t> ribozyme switches in human cells for unlocalized <t>(2MS2mut-DsRed),</t> nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.
    Dna Fragment Encoding 2ms2mut, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+fragment+encoding+2ms2mut/DNA/pmc04231745-77-1-13
    Average 99 stars, based on 1 article reviews
    dna fragment encoding 2ms2mut - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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    Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of MS2-responsive ribozyme switches in human cells for unlocalized (2MS2mut-DsRed), nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.

    Journal: Nucleic Acids Research

    Article Title: Protein-responsive ribozyme switches in eukaryotic cells

    doi: 10.1093/nar/gku875

    Figure Lengend Snippet: Effect of ligand localization on protein-responsive ribozyme switch activity. ( A ) Gene-regulatory activity of MS2-responsive ribozyme switches in human cells for unlocalized (2MS2mut-DsRed), nuclear localized (NLS-2MS2mut-DsRed) and cytoplasmic localized (2MS2mut-DsRed-NES) ligand. Reported BFP values are the geometric mean ± SD from biological duplicates and normalized to the non-cleaving control (sTRSVctrl). ( B and C ) Gene-regulatory activity of (B) MS2-A11 and (C) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of doxycycline concentration (0–50 μg/l). Reported BFP values are the geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). ( D and E ) Gene-regulatory activity of (D) MS2-A11 and (E) MS2-B1 for unlocalized, nuclear localized and cytoplasmic localized ligand as a function of ligand concentration. Ligand levels are reported as the DsRed geometric mean ± SD from biological duplicates, except MS2-B1 at 0.8 μg/l, unlocalized (singlet). BFP and DsRed levels are reported for stably integrated constructs encoding the indicated ribozyme switch and ligand expression cassettes at the indicated doxycycline or ligand levels.

    Article Snippet: A DNA fragment encoding 2MS2mut (MS2 V75E/A81G head-to-tail fused dimer) was synthesized by GeneArt (Life Technologies) and inserted into pCS2595 between NotI/ApaI to form pCS2686.

    Techniques: Activity Assay, Control, Concentration Assay, Stable Transfection, Construct, Expressing